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# Analysis of amino acids by electrophoresis

Method The amino acids can be separated according their $pI$ ( →    isoelectric point ) by electrophoresis:

The mixture to be characterized is deposited at 0. In the electric field, amino acids 1 and 2 migrate differently.

Amino Acids - having values of $pI$ greater than the $pH$ of the electrophoresis buffer have a positive overall charge: They migrate to the negative electrode. - having values of $pI$ less than the buffer $pH$ have a rather negative overall charge: They migrate to the positive electrode.

Example